雜志名稱：Advanced Science
影響因子：14.3
文章題目：An Integrated Whole‐Process Repair System with Programmed Regulation of Healing Performance Facilitates Urethral Wound Restoration and Scarless Reconstruction
DOI: https://doi.org/10.1002/advs.202409930
第一作者：Wenzhuo Fang, Ying Wang, Kaile Zhang, Ming Yang, Meng Liu, Yangwang Jin, Xianjie Xiu, Yuhui Wang, Zhenwei Yu, Ranxing Yang, Qiang Fu
作者單位：上海交通大學醫(yī)學院附屬第六人民醫(yī)院
引用YOBIBIO產品：
U96-1640E Rat VEGF/VEGFA ELISA Kit
U96-2901E Rat PDGF-BB ELISA Kit
U96-2054E Rat EGF ELISA Kit
U96-1738E Rat bFGF/FGF2 ELISA Kit
U96-2401E Rat HGF ELISA Kit
文章摘要：
The harsh microenvironment of the urethral injury often carries high risks of early undesirable healing as well as late scar tissue formation. Indeed, the infection and inflammatory response in the early stages as well as blood vessel formation and tissue regeneration in the later stages fundamentally impact the outcomes of urethral wound healing. Innovatively, an integrated whole‐process repair hydrogel (APF/C/L@dECM) is designed. After rigorous testing, it is found that hydrogels formed by hydrophobic association and double cross‐linking of amide bonds can procedurally regulate wound healing in all phases to match the repair process. In rabbit models of urethral wound, APF/C/L@dECM hydrogel can achieve scarless reconstruction with significantly better results than other hydrogels. Noteworthily, multi‐stage mechanistic explorations reveal the expression profiles of inflammation, vascularization, and extracellular matrix secretion‐related genes in wound tissue at different times. In summary, this study develops an overall treatment for urethral injury through a whole‐process repair system that promotes healthy healing of urethral wounds and prevents the formation of scar tissue.
尿道損傷的惡劣微環(huán)境通常具有早期不良愈合和晚期疤痕組織形成的高風險。事實上，早期的感染和炎癥反應以及后期的血管形成和組織再生從根本上影響尿道傷口愈合的結果。創(chuàng)新性地設計了一種一體化全過程修復水凝膠 （APF/C/L@dECM）。經過嚴格測試，發(fā)現由疏水締合和酰胺鍵雙交聯(lián)形成的水凝膠可以在所有階段程序上調節(jié)傷口愈合以匹配修復過程。在尿道傷口的兔模型中，APF/C/L@dECM 水凝膠可以實現無疤痕重建，效果明顯優(yōu)于其他水凝膠。值得注意的是，多階段機制探索揭示了傷口組織中炎癥、血管形成和細胞外基質分泌相關基因在不同時間的表達譜。綜上所述，本研究通過全程修復系統(tǒng)開發(fā)了一種整體治療尿道損傷的方法，該系統(tǒng)可以促進尿道傷口的健康愈合并防止疤痕組織的形成。
