雜志名稱：Bioactive Materials
影響因子：18.0
文章題目：Biguanide chitosan microneedles with cell-free DNA scavenging ability for psoriasis therapy
DOI：https://doi.org/10.1016/j.bioactmat.2023.11.015
第一作者：Zihao Liu, Yu Wang, Yuhan Zhang, Liufu Hu, Bozhi Chen, Yang Li, Xindong Guo, Bingran Yu, Fu-Jian Xu
作者單位：北京化工大學(xué)
引用YOBIBIO產(chǎn)品：
U96-1494E Mouse IL-1β ELISA Kit
文章摘要：
High levels of cell-free DNA (cfDNA) induce psoriasis. Currently, the treatment of psoriasis has the disadvantages of penetration difficulty, suppression of normal immunity, and skin irritation. In this study, biguanide chitosan microneedles (BGC-MNs) were prepared to treat psoriasis by removing cfDNA from the dermis through the skin barrier. The effects of chitosan with different bisguanidine contents on DNA-binding capacity, biocompatibility, and inflammation inhibition were compared, revealing that chitosan containing 20% bisguanidine (BGC2) was found to have the best overall performance. BGC2 effectively cleared cfDNA and inhibited the production of inflammatory factors. BGC-MN made from BGC2 had good mechanical and solubility properties. BGC-MNs cleared cfDNA, reduced the level of inflammatory factors in the dermis, and exerted a good therapeutic effect on mice with psoriasis. These results suggested that BGC-MNs provided a new approach to treating psoriasis in terms of scavenging cfDNA and exerting anti-inflammatory effects.
高水平的游離 DNA (cfDNA) 會誘發(fā)牛皮癬。目前銀屑病的治療存在滲透困難、正常免疫力受到抑制、皮膚刺激等缺點。在這項研究中，制備了雙胍殼聚糖微針（BGC-MNs），通過皮膚屏障去除真皮中的 cfDNA 來治療牛皮癬。比較了不同雙胍含量的殼聚糖對DNA結(jié)合能力、生物相容性和炎癥抑制的影響，發(fā)現(xiàn)含有20%雙胍的殼聚糖（BGC2）具有最佳的綜合性能。 BGC2有效清除cfDNA并抑制炎癥因子的產(chǎn)生。由BGC2制成的BGC-MN具有良好的機(jī)械性能和溶解性能。 BGC-MNs可清除cfDNA，降低真皮內(nèi)炎癥因子水平，對銀屑病小鼠發(fā)揮良好的治療作用。這些結(jié)果表明，BGC-MNs 在清除 cfDNA 和發(fā)揮抗炎作用方面為治療銀屑病提供了一種新方法。
