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Anti-β- tubulin Monoclonal Antibody 

PRODUCT NAME：Anti-β-tubulin Monoclonal Antibody 

CATALOG NUMBER：U33-290A 

LOT NUMBER：U33022 

ISOTYPE：Mouse IgG1

Clone NO：1B20 

SOURCE：Mouse Ascites  

QUANTITY：400ul 

DESCRIPTION：Monoclonal antibody is produced by immunizing mouse with synthetic peptide [(ESNMNDLVSEYQQYQDATA], coupled to KLH. 

REACTIVITY：human, mouse, African green monkey 

APPLICATIONS：Western Blot, Immunoprecipitation 

BUFFER：1×PBS with 50% glycerol 

PROTEIN CONCENTRATION：0.5 mg/ml 

APPLICATION NOTES：Western Blot: 0.5μg/ml 

STORAGE/STABILITY：Antibody is recommended being stored at -20℃. Avoid repeated freeze-thaw cycle.

 Background: 

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.