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Monoclonal Anti-His Tag-Peroxidase (HRP) Conjugated

PRODUCT NAME：Monoclonal Anti-His Tag-Peroxidase (HRP) Conjugated      

CATALOG NUMBER：U33-421A-S

LOT NUMBER：U33022

ISOTYP：Mouse IgG1 

Clone NO：1B20

SOURCE： Mouse Ascites

PURIFICATION：Protein A purified

QUANTITY：100μl

DESCRIPTION：Monoclonal antibodies are produced by immunizing mouse with synthesized polypeptide 6 x His, coupled with KLH. This antibody can recognize the His-tag (HHHHHH) fused to either the amino or carboxy terminal of targeted protein.

APPLICATION：Western Blot

REAGENT：1×PBS with 50% glycerol

PROTEIN CONCENTRATION：approximately 1mg/ml

RECOMMENDED ANTIBODY DILUTION：Western Blot: 1:4000

STORAGE/STABILITY：Antibody-HRP is recommended being stored at -20℃.

Background:

Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibody have been widely used in study of protein expression in various systems. Our anti-His Tag-peroxidase conjugated monoclonal antibody provides a simple solution to detect the expression of protein with a His tag.