雜志名稱：Journal of Food Biochemistry
影響因子：3.5
文章題目：Curcumin-Loaded Mesenchymal Stem Cell-Derived Exosomes: A Potential Effective Strategy for NAFLD
DOI：https://doi.org/10.1155/jfbc/6134296
第一作者：Ruixi Luo ,Jing Zhang ,Peng Chen ,La Wang ,Wenjia Wang ,Ping Wang ,Xing Zhao ,Weiyi Tian
作者單位：貴州中醫(yī)藥大學(xué)、貴州醫(yī)科大學(xué)
引用YOBIBIO產(chǎn)品：
U96-1511E Mouse IL-6 ELISA Kit
文章摘要：
Te aim of this study was to construct curcumin-loaded mesenchymal stem cell-derived exosomes (Cur-exos) and explore their efects on nonalcoholic fatty liver disease (NAFLD) models both in vitro and in vivo. Cur-exos were prepared using three common drug-loading techniques—incubation, sonication, and freeze–thaw cycles. Among these, Cur-exos prepared via repeated freeze–thawing demonstrated higher encapsulation efciency and drug-loading rates than those produced by other methods. In vitro, Cur-exos exhibited enhanced stability, were efciently taken up by hepatocytes, and mitigated palmitic acid (PA)–induced lipotoxicity in HepG2 cells. Moreover, Cur-exos signifcantly ameliorated liver damage in NAFLD model mice, reduced infammation, decreased reactive oxygen species (ROS) levels, and mitigated endoplasmic reticulum (ER) stress. Additionally, Curexos efectively regulated lipid metabolism disorders and improved impaired glucose tolerance. Overall, we demonstrate that, compared with free curcumin, Cur-exos signifcantly improve curcumin stability and ofer superior therapeutic efects in NAFLD mouse models. Tis study provides a novel approach for developing treatments for NAFLD.
本研究的目的是構(gòu)建載有姜黃素的間充質(zhì)干細(xì)胞衍生外泌體（Cur-exos），并探索其在體外和體內(nèi)對非酒精性脂肪肝�。∟AFLD）模型的影響。Cur-exos采用三種常見的藥物載體制備技術(shù)——孵育、超聲波處理和凍融循環(huán)。其中，通過反復(fù)凍融制備的Cur-exos表現(xiàn)出比其他方法更高的包封效率和載藥率。在體外實(shí)驗(yàn)中，Cur-exos表現(xiàn)出更高的穩(wěn)定性，被肝細(xì)胞有效攝取，并減輕了棕櫚酸（PA）引起的HepG2細(xì)胞脂毒性。此外，Cur-exos顯著改善了NAFLD模型小鼠的肝損傷，減少了炎癥，降低了活性氧（ROS）水平，并減輕了內(nèi)質(zhì)網(wǎng)（ER）應(yīng)激。此外，Cur-exos有效調(diào)節(jié)了脂質(zhì)代謝紊亂，改善了受損的葡萄糖耐受性�？傮w而言，與游離姜黃素相比，Cur-exos顯著提高了姜黃素的穩(wěn)定性，并在NAFLD小鼠模型中提供了更優(yōu)的治療效果。本研究提供了一種新的方法。
